A plethora of boological processes occurs al the interface between the cytoplasmic milieu and the fhembrane, These include for insiance transport of (macrojmolecules and membrane re-shaping mi the Context of subcellular trafic and cell division Giant Unilamedlar Vesicles (GUVs) have been used for decades a5 a tool bo reconstitute and study these processes in vitro. Yet, we are still very limited im our abiity to induce GUVs re-shaping and to rehably contro! protein reconstitubon Here, introduce a new approachemical io generate GUVs called Synthetic Membrane Shaper (SMS) 1 and illustrate is potenbal in addressing these challenges. The SMS allows to encapsulate a wide range of molecules. and particles, inctuding bacteria and large DNA molecules 7, to reconstitute Wansmembrane pores to study transport Processes 3 and to oblain complex membrane shapes in a controled fashion 7 which led to the reconstiubon of synthetic cell division im vitno 4 Finaify, will introduce our ongoing research on reconstitution of double membranes as a novel tool to Study Membrane rearrangement mduced by Sars-CoVv-2 5. 1 De Franceschi et. al Synthetic Membrane Shaper for Controtied Liposome Deformation ACS Nang 2022 2. Van de Cauter ét al Ophmtized eDICE tar Effiment Reconstitution of Biological Systems m Giant Unilamellar Vescies. ACS Synth Biol 2021 3 Fragasso & De Franceschi el al Reconsttutton of Ulttrawide DNA, Origami Pores in Liposomes. for Transmembrane Transport of Macromolecules. ACS Nano. 2024 4 De Franceschi ef al Dynamm. A as a one-component drasion machmery for synthebc celts. Nat Nanotechnol 2023 6 Ricciardi et al. The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle. Nature. 2022
About the speaker
Dr. De Franceschi holds a Sonata Bis grant and he is a Group Leader at the International Institute of Molecular Mechanisms and Machines (IMOL), Polish Academy of Sciences, in Warsaw. He performed postdoctoral work as a Marie Curie Fellow at the Curie Institute in Paris and the Institute of Structural Biology in Grenoble, and completed a second postdoc at Delft University of Technology
